FDA Coronavirus Testing Basics
https://www.fda.gov/media/138094/download
Since no quantified virus isolates of the 2019-nCoV were available
for CDC use at the time the test was developed and this study conducted,
assays designed for detection of the 2019-nCoV RNA were tested with
characterized stocks of in vitro transcribed full length RNA (N gene;
GenBank accession: MN908947.2) of known titer (RNA copies/%u03BCL)
spiked into a diluent consisting of a suspension of human A549 cells and
viral transport medium (VTM) to mimic clinical specimen.
https://www.fda.gov/media/134922/download
"They didn't isolate the virus; that's the issue"
https://www.bitchute.com/video/mGYefByrG0h0/
Original footage from the "outbreak"
https://www.dailymail.co.uk/video/china/video-2094890/Video-Dramatic-footage-shows-people-collapsing-suddenly-Wuhan-city.html
Identification of Coronavirus Isolated from a Patient in Korea with COVID-19
Nasopharyngeal
and oropharyngeal swab and sputum samples were collected from
symptomatic patients to detect SARS-CoV-2 by real-time reverse
transcriptase (RT)-PCR. RNA was extracted from clinical samples
https://archive.md/B9aUt
We designed 37 pairs of nested PCRs spanning the genome on the basis
of the coronavirus reference sequence (GenBank accession no. NC045512).
We extracted nucleic acid from isolates and amplified by using the 37
individual nested PCRs. We used positive PCR amplicons individually for
subsequent Sanger sequencing and also pooled them for library
preparation by using a ligation sequencing kit
https://archive.md/yMxyz
We simultaneously obtained two nasopharyngeal samples with flocked
swabs; one was used for the antigen test and the other for real-time
reverse transcription PCR (RT-PCR). Using the results of real-time
RT-PCR as a reference, the performance of the antigen test was
evaluated.
https://archive.md/091m5#selection-2677.223-2677.504
Antigen-based tests for SARS-CoV-2: Virus culture was attempted on all antigen-positive or real-time RT-PCR-positive specimens.
https://archive.md/sSFNP
Rapid antigen test: Detects easy-to-find surface markers on the
outside of the virus and avoids extraction and amplification steps.
Researchers or clinicians collect samples from easy-to-reach areas (like
the nasal passage) where the virus tends to replicate the most. the
test may yield false negatives if the viral protein production is low or
if there is not enough virus replication in the sampled area.
https://archive.md/k0l3m
Firstly it is incorrect to say that the virus that causes Covid-19
has not been isolated. Isolating a virus means taking a pure sample of a
virus from an infected being so it can be studied. There are numerous
reports of the virus being isolated by teams around the world.
https://archive.md/z32W9
The outbreak sent researchers around the world racing to isolate
laboratory specimens of the virus that causes COVID-19. The virus was
later named severe acute respiratory syndrome coronavirus 2, or
SARS-CoV-2.
https://archive.md/BVuiR
Nasopharyngeal and oropharyngeal swab and sputum samples were
collected from symptomatic patients to detect SARS-CoV-2 by real-time
reverse transcriptase (RT)-PCR. RNA was extracted from clinical samples
https://archive.md/B9aUt
The evaluation of a newly developed antigen test
(QuickNavi%u2122-COVID19 Ag) for SARS-CoV-2: A prospective observational
study in Japan
https://archive.md/091m5
Dr. Rizza says, "There are other ways to determine if somebody has
been previously exposed to COVID-19, which is through a serology blood
test." She adds that, "current infection can also be diagnosed by using a
blood test which looks for certain proteins or antigens. But the most
sensitive test for active infection right now, is looking for that
genetic material."
https://archive.md/mTFkV
COVID-19 antibody testing, also known as serology testing, is a blood
test that's done to find out if you've had a past infection with
SARS-CoV-2, the virus that causes coronavirus disease 2019 (COVID-19).
An antibody test can't determine whether you're currently infected with
the COVID-19 virus.
https://archive.md/iqidN
Serology uses PCR
miRNAs can be quantified using quantitative Real Time PCR, similar to traditional DNA samples.
https://archive.md/AbiSG#selection-705.173-709.0
Disease Burden of Influenza (CDC) (millions of symptomatic cases per
year, hundreds of thousands of hospitalizations and thousands of deaths)
https://archive.md/nqgIZ
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